Nucleic Acids
Fluorescence-based determination and detection of DNA and RNA using solution fluorimetry, gel and capillary electrophoresis, fluorescence microscopy, and flow cytometry.
Fluorescence enables the detection of binding, aggregation and conformational changes in biomacromolecules. We work with monomethine, trimethine, dioxaborine and other dye classes and compare their spectral responses in different biological systems.
Research Targets
Proteins and Conformational Changes
Probes for detecting proteins, partially denatured conformations and β-sheet aggregates.
Long-wavelength cyanine dyes for biodetection and bioimaging.
Development of long-wavelength cyanine dyes for highly sensitive fluorescence detection of biomolecules, cellular structures, and parameters of the biological microenvironment.
Biomedical Diagnostics
We create dyes and molecular probes for proteins, nucleic acids and biomolecular aggregates and study their interaction mechanisms.
Research Workflow
Molecular Design
Selection of the chromophore, substituents and spectral range for a specific biological application.
Dye Collection and Synthesis of New Structures
Use of more than a thousand dyes from our in-house collection and, depending on the project objective, synthesis of new series of related structures for systematic study and comparison of their properties
Spectral Characteristics
Absorption, emission, quantum yield and signal changes in the presence of the target biomacromolecule.
Validation in an Analytical System
Assessment of selectivity, sensitivity and suitability for a specific biomedical application.
Key Results and Applications
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DNA / RNA and Protein Probes
Dyes for fluorescence detection of nucleic acids and proteins
Protein Detection
Long-wavelength probes for proteins, conformational changes and β-sheet aggregates.
Diagnostic Formats
Fluorescent reagents for PCR and analytical systems.
Research Team
Cyanine and other fluorophore classes tailored to specific molecular targets and analytical tasks.
Learn morePublications
The developed probes, dyes and detection methods are presented in the Department’s publications.
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